A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

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In this study, we developed a novel method for the detection of Vibrio alginolyticus, utilizing the specific recognition of an aptamer for V.alginolyticus and signal amplification via hybridization chain reaction (HCR) and horseradish-peroxidase-conjugated streptavidin.The proposed HCR-based multivalent aptamer (multi-Apt) amplifier allows for popularfilm.blog sensitive detection of V.alginolyticus in a linear range from 10 to 107 CFU/mL.The linear equation is y = 747.

5x + 126.2, R2 = 0.986, and the limit of detection (LOD) is 3 CFU/mL.Seawater and freshwater samples were utilized in the spike recovery experiment, yieldng a recovery rates ranging from 94.3% to 108.

8%.The relative standard deviation (RSD) for all samples is below 6.73%.Taken together, the proposed method has great potential for application in monitoring here of V.alginolyticus in aquaculture environments.